Diffusional and electrostatic effects on apparent kinetic parameters of reactions catalyzed by enzyme immobilized on the external surface of a support |
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| Institution: | 1. Chemistry Department, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi Arabia;2. Photochemistry Department, National Research Centre, Dokki, 12622 Giza, Egypt;3. Department of Physics, Faculty of Science, Suez University, Suez 43518, Egypt;4. Faculty of Nanotechnology For Postgraduate Studies, Cairo University, El?Sheikh Zayed 12588, Egypt;5. Institute of Nanoscience & Nanotechnology, Kafrelsheikh University, 33516 Kafrelsheikh, Egypt |
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| Abstract: | Diffusional and electrostatic effects on the apparent maximum reaction rate Vmapp and the apparent Michaelis constant Kmapp were investigated theoretically for a system in which an enzyme immobilized on the external surface of a solid support catalyzes a reaction according to Michaelis-Menten kinetics. In such a system, the dependence of Vmapp and Kmapp on the substrate concentration can be expressed analytically. When the support and substrate carry charges of the same sign, resulting in a repulsive force between them, both Vmapp and Kmapp decrease with increasing substrate concentration, but they never decrease below the respective intrinsic values. On the other hand, when the support and substrate carry charges of opposite sign and therefore an attractive force occurs, Vmapp decreases towards its intrinsic value, while Kmapp decreases to values below its intrinsic value in the region of high substrate concentration. |
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