Observation of microorganism colonies using a scanning-laser-beam pH-sensing microscope |
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| Affiliation: | 1. The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567 Japan;2. Nakano Central Research Institute, Nakano Vinegar Co. Ltd., 2-6 Nakamura-cho, Handa, Aichi 475, Japan;1. Institute of Medical Engineering, School of Basic Medical Sciences, Health Science Center, Xi''an Jiaotong University, Xi''an, 710061, China;2. School of Electronic and Information Engineering, Department of Biophysics, Xi''an Jiaotong University, Xi''an, 10049, China;3. School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK;4. Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China;5. State Key Laboratory of Powder Metallurgy, School of Materials Science and Engineering, Central South University, Changsha, 410083, China;1. School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China;2. Guangxi Key Laboratory of Marine Environmental Science, Guangxi Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China;3. Guangxi Special Equipment Inspection and Research Institute Guiding Branch, Guilin, Guangxi 541004, China;1. Institute of Medical Engineering, School of Basic Medical Science, Health Science Center, Xi''an Jiaotong University, Xi''an 710061, China;2. Department of Biochemistry and Genetics, School of Medicine, Zhejiang University, Hangzhou 310058, China;3. Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China;1. School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London E1 4NS, UK;2. School of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China;3. School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK;1. School of Electronics and Information, Northwestern Polytechnical University, Xi''an, Shaanxi, 710072, PR China;2. The Research & Development Institute of Northwestern Polytechnical University in Shenzhen, Shenzhen, 518057, PR China;3. School of Mechanical and Electrical Engineering, Zhoukou Normal University, Zhoukou, Henan, 466001, PR China |
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| Abstract: | The extracellular pH-distribution of colonies of Saccharomyces cerevisiae (yeast) and Escherichia coli (E. coli) were observed using a newly-developed scanning-laser-beam pH-sensing microscope. Colonies were incubated either on top of agarose plates or between the pH-sensing surface and the agar. In the latter case, colony growth was observed in-situ. The colonies could be observed within a period as short as 8 h for E. coli. The pH-distribution profiles by the colonies were found to be very sharp, in agreement with simulation results. |
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