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Preparation of artificial seeds using cell aggregates from horseradish hairy roots encapsulated in alginate gel with paraffin coat |
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| Institution: | 1. National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China;2. Key Laboratory of Natural Products Research and Development of Li Folk Medicine of Hainan Province, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China;3. Experimental Center of Tropical Forestry, Chinese Academy of Forestry/Guangxi Youviquan Forest Ecosvstem Research Station, Pingxiang, Guangxi, 532600, China;1. Division of Genomic Resources, ICAR-NBPGR, New Delhi, India;2. Faculty of Science, Jamia Hamdard University, Hamdard Nagar, Delhi, India |
| Abstract: | Cell aggregates (CA) derived from horseradish hairy roots were used as inclusion materials for artificial seeds by entrapping them in alginate gel capsules. Regeneration of adventitious roots from the encapsulated CA was suppressed by covering the capsules with thin paraffin coats throughout storage of the capsules. The CA in coated capsules stored at 25°C up to 60 d retained root regeneration potential comparable to that of CA which were not subjected to storage. Plantlets were formed from the roots emerging from the CA in the capsules cultured under light condition. |
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