Biosynthetic Pathways of Glycerol Accumulation under Salt Stress in Aspergillus nidulans |
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| Institution: | 1. Laboratory for Yeast Molecular and Cell Biology, The Research Center of Fermentation Technology, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, Shandong, China;2. College of Life Sciences, Huaibei Normal University, Huaibei 235000, Anhui, China;1. Commonwealth Scientific and Industrial Research Organization (CSIRO) Agriculture, Queensland Bioscience Precinct, Brisbane, Queensland 4067, Australia;2. University of Queensland (UQ), School of Agriculture and Food Sciences, St. Lucia, Queensland 4067, Australia;3. University of Western Australia, School of Plant Biology, Crawley, Western Australia 6009, Australia;4. CSIRO Agriculture, Black Mountain, Canberra, Australian Capital Territory 2601, Australia;5. UQ, Queensland Alliance for Agriculture & Food Innovation, St. Lucia, Brisbane, Queensland 4067, Australia;1. Commonwealth Scientific and Industrial Research Organization (CSIRO) Agriculture Flagship, Queensland Bioscience Precinct, Brisbane, Queensland 4067, Australia;2. University of Queensland, School of Agriculture and Food Sciences, St. Lucia, Queensland 4067, Australia;3. University of Western Australia, School of Plant Biology, Crawley 6009, Western Australia, Australia;4. CSIRO Agriculture Flagship, Black Mountain, Canberra, Australian Capital Territory 2601, Australia;5. Queensland Alliance for Agriculture & Food Innovation, The University of Queensland, St. Lucia, Brisbane, Queensland 4067, Australia;1. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 10010, PR China;2. University of Chinese Academy of Sciences, Beijing 100049, PR China;1. Departamento de Química Orgánicay Química Inorgánica, Universidad de Alcalá, Facultad de Farmacia, Autovía A2, 33.600, 28871, Alcalá de Henares, Madrid, Spain;3. Departamento de Quimica Organica, Universidad de Cordoba, Campus de Rabanales, Edificio Marie Curie (C-3), Ctra Nnal IV-A, Km 396, 14014 Cordoba, Spain |
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| Abstract: | Redkar, R. J., Locy. R. D., and Singh, N. K. 1995. Biosynthetic pathways of glycerol accumulation under salt stress in Aspergillus nidulans. Experimental Mycology 19, 241-246. A culture of Aspergillus nidulans (FGSC 359) was gradually adapted for growth in media containing up to 2 M NaCl or was exposed to a salt shock with 2 M NaCl. The intracellular glycerol level increased by about 7.9-fold in salt-adapted and 2.4-fold in salt-shocked cultures when compared to the unadapted culture. The biosynthetic pathway involved in the accumulation of glycerol was investigated under long-term salt adaptation and short-term salt shock. Glycerol 3-phosphate dehydrogenase (EC 1.1.1.8) was induced 1.4-fold in salt-shocked but not in salt-adapted cultures. An alternate enzymatic pathway involving glycerol dehydrogenase (NADP+-dependent) utilizing dihydroxyacetone (DHA) and/or DL-glyceraldehyde (DL-GAD) was induced by NaCl. DHA-dependent glycerol dehydrogenase activity was induced about 6.3-fold in salt adapted and 1.35-fold in salt-shocked cultures, while DL-GAD-dependent activity was induced about 6.1-fold in salt-adapted and 1.2-fold in salt shocked cultures. However, the level of glycerol dehydrogenase activity with DL-GAD as substrate was 7% of the DHA-dependent activity. We conclude that a salt-inducible NADP+-dependent glycerol dehydrogenase activity electrophoretically indistinguishable from previously described glycerol dehydrogenase I results in glycerol accumulation in salt-stressed A. nidulans. |
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