5-Azacytidine enhances transient expression of a transfected gene in cultured mammalian cells |
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| Institution: | 1. Department of Medicine, Section of Hematology-Oncology, Boston University School of Medicine, Boston, MA 02118, USA;2. Department of Medicine, Section of Biomedical Genetics, Boston University School of Medicine, Boston, MA 02118, USA;3. Research and Development Department, Central Blood Institute, Blood Service Headquarters, Japanese Red Cross Society, Tokyo, Japan;4. Cell Engineering Division, RIKEN BioResource Center, Ibaraki, Japan;5. Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, MA 02118, USA |
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| Abstract: | The level of transient expression of the Escherichia coli β-galactosidase (β-Gal) gene transfected into three mammalian cell lines was enhanced by culturing the cells with 1–5 μM 5-azacytidine (5-azaC) after the transfection. This enhancement was similarly observed in four different transfection procedures tested. The enhancement of β-Gal expression was primarily due to the increase in the level of its mRNA. Since 5-azaC did not exhibit any obvious effect on the levels of endogenous gene products examined, it is suggested that 5-azaC may preferentially affect the transient expression of an exogenous gene. |
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