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l-Serine synthesis using the resting Hyphomicrobium sp. NCIB10099 cells under suppressive conditions for l-serine-degrading activity
Affiliation:1. Department of Food and Nutrition, Faculty of Agriculture, Kinki University, Naka-machi, Nara 631, Japan;2. Department of Biotechnology, Faculty of Engineering, Tottori University, Tottori 680, Japan;1. Department of Microbiology, Soochow University, 70, LinXi Rd., Shinlin Dist., Taipei, 11102, Taiwan;2. Graduate Institute of Environmental Engineering, National Central University, 300, Zhongda Rd., Zhongli Dist., Taoyuan City, 32001, Taiwan;1. Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu, 610041, China;2. University of Chinese Academy of Sciences, Beijing, 100049, China;1. College of Environment and Ecology, Hainan University, Renmin Road, Haikou 570228, China;2. MOE Key Laboratory of Pollution Process and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, No. 94 Weijin Road, Nankai District, Tianjin 300071, China;3. Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan
Abstract:l-Serine-degrading activity could be suppressed by controlling the methanol concentration in l-serine synthesis using resting Hyphomicrobium sp. NCIB10099 cells. Fifty-three mg/ml of l-serine were produced from 100 mg/ml of glycine and 104 mg/ml of methanol. It is believed that l-serine-degrading activity contributes to the reverse serine hydroxymethyltransferase reaction.
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