Effects of insulin-like growth factor-I,estrogen, glucocorticoid,and transferrin on the mRNA contents of ovalbumin and conalbumin in primary cultures of quail (Coturnix coturnix japonica) oviduct cells |
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| Institution: | 1. Department of Neurology, Brigham and Women''s Hospital, Boston, MA, USA;2. Ann Romney Center for Neurologic Diseases, Brigham and Women''s Hospital, Boston, MA, USA;3. Harvard Medical School, Boston, MA, USA;4. Probiodrug AG, Halle (Saale), Germany;5. Harvard NeuroDiscovery Center NeuroBehavior Laboratory Core, Harvard Institutes of Medicine, Boston, MA, USA;1. Stem Cell Research Center, Stem Cells and Regenerative Medicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran;2. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;3. Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;4. Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;5. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran;6. Gene Yakhteh Keyhan (Genik) Company (Ltd), Pharmaceutical Biotechnology Incubator, Tabriz University of Medical Sciences, Tabriz, Iran;7. ARTAN1100 Startup Accelerator, Tabriz, Iran;8. Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;9. Department of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;10. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;11. Stem Cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran;12. Andam Baft Yakhteh (ABY) Company (Ltd), Tehran, Iran;13. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran;14. Medical Nanotechnology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;15. Department of Tissue Engineering and Applied Cell Science, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;p. Zist Andam Yakhteh Azerbaijan (ZAYA) Company (PHT), Medical Instrument Technology Incubator, Tabriz University of Medical Sciences, Tabriz, Iran;q. Nanotechnology and Regenerative Medicine Commercialization Centre (Ltd), The London Innovation Bio Science Centre, London NW1 0NH, United Kingdom;r. Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran;s. HealthNBICS Group, Convergence of Knowledge and Technology to the benefit of Society Network (CKTSN), Universal Scientific Education and Research Network (USERN), Tabriz, Iran;1. Department of Cardiology, General Hospital “Prim.Dr. Abdulah Nakas”, Sarajevo, Bosnia and Herzegovina;2. Department of Pharmacology, Sarajevo Medical School, Sarajevo School of Science and Technology, Sarajevo, Bosnia and Herzegovina;1. Department of Urology, Nihon University School of Medicine, Japan;2. Department of Anti-Aging Medicine, Graduate School of Medicine, The University of Tokyo, Japan;3. Department of Geriatric Medicine, Graduate School of Medicine, The University of Tokyo, Japan;4. Division of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Japan;5. Division of General Medicine, Department of Medicine, Nihon University School of Medicine, Japan;6. Department of Urology, Graduate School of Medicine, The University of Tokyo, Japan;1. Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Box 7028, SE-750 07 Uppsala, Sweden;2. Department of Chemical Science, University of Catania, Viale Andrea Doria 6, I-95125 Catania, Italy |
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| Abstract: | The effects of estrogen, dexamethasone, insulin-like growth factor-I (IGF-I), and transferrin on the messenger RNA (mRNA) contents of ovalbumin and conalbumin in primary cultures of quail oviduct cells were investigated. In the absence of one of the above hormones or factors, a decrease in ovalbumin mRNA was prominent. In particular, removal of IGF-I and transferrin caused a significant effect. Studies using a combination of estrogen, dexamethasone, IGF-I and transferrin indicated that IGF-I cooperates with estrogen or dexamethasone and transferrin works with dexamethasone. Specifically, IGF-I enhanced ovalbumin synthesis or increased cellular ovalbumin mRNA content depending on its concentration in the medium in the presence of estrogen. However, the effects of estrogen, dexamethasone, IGF-I, and transferrin were not similarly observed with conalbumin mRNA. These results show that ovalbumin synthesis is controlled by estrogen or glucocorticoid with IGF-I or transferrin and that cellular ovalbumin mRNA content is also regulated by these hormones or transferrin. In contrast, conalbumin synthesis and cellular content of conalbumin mRNA are not affected by these hormones under the conditions of the present study. |
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