Photosensitized covalent organic framework as a light-induced oxidase mimic for colorimetric detection of uric acid |
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Authors: | Meng Ping Wang Hui Han Li Ting Wu Sai Jin Xiao Guang Zhou Liu Li Zhang |
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Institution: | 1. School of Chemistry and Material Science, East China University of Technology, Nanchang, China
Contribution: ?Investigation, Methodology, Writing - original draft;2. School of Chemistry and Material Science, East China University of Technology, Nanchang, China
Contribution: Data curation, ?Investigation, Methodology, Validation;3. School of Chemistry and Material Science, East China University of Technology, Nanchang, China
Contribution: ?Investigation, Methodology;4. School of Chemistry and Material Science, East China University of Technology, Nanchang, China;5. School of Chemistry and Material Science, East China University of Technology, Nanchang, China
Contribution: Methodology, Validation;6. School of Chemistry and Chemical Engineering, Nanchang University, Nanchang, China |
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Abstract: | As large numbers of people are suffering from gout, an accurate, rapid, and sensitive method for the detection of gout biomarker, uric acid, is important for its effective control, diagnosis, and therapy. Although colorimetric detection methods based on uricase have been considered, they still have limitations as they produce toxic H2O2 and are expensive and not stable. Here, a novel uricase-free colorimetric method was developed for the sensitive and selective detection of uric acid based on the light-induced oxidase-mimicking activity of a new photosensitized covalent organic framework (COF) (2,4,6-trimethylpyridine-3,5-dicarbonitrile–4-2-(4-formylphenyl)ethynyl]benzaldehyde COF DCTP–EDA COF]). DCTP–EDA COF has a strong ability to harvest visible light, and it could catalyze the oxidation of 1,4-dioxane, 3,3′,5,5′-tetramethylbenzidine under visible light irradiation to produce obvious color changes. With the addition of uric acid, however, the significant inhibition of the oxidase-mimicking activity of DCTP–EDA COF remarkably faded the color, and thus uric acid could be colorimetrically detected in the range of 2.0–150 μM with a limit of detection of 0.62 μM (3σ/K). Moreover, the present colorimetric method exhibited high selectivity; uric acid level in serum samples was successfully determined, and the recoveries ranged from 96.5% to 105.64%, suggesting the high accuracy of the present colorimetric method, which demonstrates great promise in clinical analysis. |
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Keywords: | colorimetric detection covalent organic framework nanozyme uric acid |
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