Comparison of liposome immune lysis assay (LILA) and enzyme-linked immunosorbent assay (ELISA) for detection of antibodies |
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| Affiliation: | 1. Department of Ophthalmology, Zhongshan Hospital, Fudan University, Shanghai 200032, China;2. Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research, Shanghai Institute of MateriaMedica, Chinese Academy of Sciences, Shanghai 201203, China;3. SIMMUOMICS Laboratory, Joint Research Laboratory of Translational “OMICS” between Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China and University of Ottawa, Canada |
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| Abstract: | Anti-α-chymotrypsinogen A antibody was assayed by both enzyme-linked immunosorbent assay (ELISA) and liposome immune lysis assay (LILA). The detection limit was slightly affected by the measurement conditions in ELISA; however, it was possible to control the detection limit and to achieve a lower level by adapting the measurement conditions in LILA. LILA is believed to offer a simple and highly sensitive method for measuring the concentration of antibody in serum. |
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