Molecular characterization of a DNA fragment carrying the basic replicon of pTUC100, the natural plasmid encoding the peptide antibiotic microcin J25 system |
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Authors: | Delgado Mónica A Salomón Raúl A |
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Affiliation: | Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas ,Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de Tucumán, Tucumán, Argentina. |
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Abstract: | The Escherichia coli plasmid pTUC100 encodes production of, and immunity to, the peptide antibiotic microcin J25. In the present study, an approximately 8-kb fragment immediately adjacent to the previously sequenced microcin region was isolated and its DNA sequence was determined. The main features of the newly characterized region are: (i) a basic replicon which is almost identical to that of the RepFIIA plasmid R100; (ii) two ORFs with 96% identity to two ORFs of unknown function on pO157, a large plasmid harbored by enterohemorragic E. coli, and a large ORF which does not show significant homology to any other reported nucleotide or protein sequence; and (iii) two intact insertion sequences, IS1294 and IS1. Sequence analysis, as well as that of the G+C content of both the 8-kb fragment and the previously sequenced microcin locus, lead us to propose that plasmid pTUC100 is a composite structure assembled from DNA elements from various sources. |
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Keywords: | Plasmid pTUC100 Microcin J25 Replicon Mosaic structure |
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