Selective changes in methionine tRNA species during development of the posterior silkglands of Bombyx mori. |
| |
Authors: | A Araya M Krauskopf M A Siddiqui |
| |
Affiliation: | Instituto de Bioquimica, Universidad Austral de Chile, Valdivia, Chile;Roche Institute of Molecular Biology, Nutley, New Jersey 07110, U. S. A. |
| |
Abstract: | Transfer RNA with methionine acceptor activity isolated from two distinct physiological stages of the developing posterior silkgland of the silkworm, , was examined. The tRNA from both stages could be fractionated on benzoylated DEAE-cellulose colum into two iso-accepting species, tRNA1Met and tRNA2Met. The molar quantity per gland of tRNA1Met species, which was also formylatable with the enzymes, increased twelve-fold as the gland differentiates to produce a large amount of a single protein, silk-fibroin. Since methionine is not a part of silk-fibroin, the preferential increase in tRNA1Met content would reflect the increased biological activity and the rapid rate of protein synthesis during the terminal differentiation of posterior silkgland. |
| |
Keywords: | To whom all correspondence should be addressed. |
本文献已被 ScienceDirect 等数据库收录! |
|