Histochemical method for demonstrating aldolase

Summary By combing the indirect method of aldolase activity of Warburg and Christian, which consisted in the measurement of reduction of DPN in the presence of glyceraldehyde-3-phosphate dehydrogenase and arsenate, with nitro-BT reduction and we could obtain the much better method of demonstrating aldolase than that of Allen and Bourne.The optimal incubating mixture was composed of 1) 10 ml 0.02 M sodium fructose-1,6-diphosphate, 2) 5 mg DPN, 3) 10 mg nitro-BT, 4) 10 ml of 0.05 M arsenate-HCl buffer (pH 7.6). Fresh frozen section, which were fixed briefly in 80% cold ethanol, gave a better staining results. The distribution of aldolase of some organs of rat and the validity and limitation of the method were described.