Kinetic studies on autohydrogenotrophic growth of <Emphasis Type="Italic">Ralstonia eutropha</Emphasis> with nitrate as terminal electron acceptor |
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Authors: | Armin Tiemeyer Hannes Link Dirk Weuster-Botz |
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Institution: | 1.Lehrstuhl für Bioverfahrenstechnik,Technische Universit?t München,Garching,Germany |
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Abstract: | Autohydrogenotrophic batch growth of Ralstonia eutropha H16 was studied in a stirred-tank reactor with nitrate and nitrite as terminal electron acceptors and the sole limiting substrates.
Assuming product inhibition by nitrite, saturation kinetics with the two limiting substrates and a simple switching function,
which allows growth on nitrite only at low nitrate concentrations, resulted in a kinetic growth model with nine model parameters.
The data of two batch experiments were used to identify the kinetic model. The kinetic model was validated with two additional
batch experiments. The model predictions are in very good agreement with the experimental data. The maximum nitrite concentration
was estimated to be 30.7 mM (total inhibition of growth). After complete reduction of nitrate, the growth rate decreases almost
to zero before it increases again because of the following nitrite respiration. The maximum autohydrogenotrophic growth rate
of Ralstonia eutropha with nitrate as a final electron acceptor (0.509 d−1) was found to be reduced by 90–95% compared to the so far reported autohydrogenotrophic growth rates with oxygen. |
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Keywords: | Ralstonia eutropha Autohydrogenotrophic Kinetic Nitrate respiration Nitrite respiration Cupriavidus necator |
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