Restoration of cutaneous pigmentation by transplantation to mice of isogeneic human melanocytes in dermal–epidermal engineered skin substitutes |
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| Authors: | Steven T Boyce Christopher M Lloyd Mark C Kleiner Viki B Swope Zalfa Abdel‐Malek Dorothy M Supp |
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| Institution: | 1. Department of Surgery, University of Cincinnati, Cincinnati, OH, USA;2. Research Department, Shriners Hospitals for Children, Cincinnati, OH, USA;3. Department of Dermatology, University of Cincinnati, Cincinnati, OH, USA |
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| Abstract: | Autologous engineered skin substitutes (ESS) containing melanocytes (hM) may restore pigmentation and photoprotection after grafting to full‐thickness skin wounds. In this study, normal hM were isolated from discard skin, propagated with or without tyrosinase inhibitors, cryopreserved, recovered into culture, and added to ESS (ESS‐P) before transplantation. ESS‐P were incubated in either UCMC160/161 or UCDM1 medium, scored for hM densities, and grafted to mice. The results showed that sufficient hM can be propagated to expand donor tissue by 100‐fold; incubation of hM in tyrosinase inhibitors reduced pigment levels but did not change hM recovery after cryopreservation; hM densities in ESS‐P were greater after incubation in UCDM1 than UCMC160 medium; hM were localized to the dermal–epidermal junction of ESS‐P; and UCDM1 medium promoted earlier pigment distribution and density. These results indicate that hM can be incorporated into ESS‐P efficiently to restore cutaneous pigmentation and UV photoprotection after full‐thickness skin loss conditions. |
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| Keywords: | cultured human melanocytes engineered skin substitutes photoprotection skin pigmentation wound closure |
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