Localization of SMAP2 to the TGN and its function in the regulation of TGN protein transport |
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| Authors: | Funaki Tomo Kon Shunsuke Ronn Roger E Henmi Yuji Kobayashi Yuka Watanabe Toshio Nakayama Keiko Tanabe Kenji Satake Masanobu |
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| Affiliation: | Institute of Development, Aging and Cancer, Graduate School of Life Sciences, Tohoku University, Sendai, Japan. |
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| Abstract: | SMAP2 is an Arf GTPase-activating protein that is located and functions on early endosome membranes. In the present study, the trans-Golgi network (TGN) was verified as an additional site of SMAP2 localization based on its co-localization with various TGN-marker proteins. Mutation of specific stretches of basic amino acid residues abolished the TGN-localization of SMAP2. Over-expression of wild-type SMAP2, but not of the mutated SMAP2, inhibited the transport of vesicular stomatitis virus-G protein from the TGN to the plasma membrane. In contrast, this transport was enhanced in SMAP2 (-/-) cells characterized by increased levels of the activated form of Arf. SMAP2 therefore belongs to an ArfGAP subtype that resides on the TGN and functions as a negative regulator of vesicle budding from the organelle. |
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