The Arabidopsis peptide kiss of death is an inducer of programmed cell death |
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Authors: | Blanvillain Robert Young Bennett Cai Yao-min Hecht Valérie Varoquaux Fabrice Delorme Valérie Lancelin Jean-Marc Delseny Michel Gallois Patrick |
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Affiliation: | Laboratoire Génome et Développement des Plantes, CNRS-UMR 5096, Université de Perpignan, Perpignan, France. |
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Abstract: | Programmed cell death (PCD) has a key role in defence and development of all multicellular organisms. In plants, there is a large gap in our knowledge of the molecular machinery involved at the various stages of PCD, especially the early steps. Here, we identify kiss of death (KOD) encoding a 25-amino-acid peptide that activates a PCD pathway in Arabidopsis thaliana. Two mutant alleles of KOD exhibited a reduced PCD of the suspensor, a single file of cells that support embryo development, and a reduced PCD of root hairs after a 55°C heat shock. KOD expression was found to be inducible by biotic and abiotic stresses. Furthermore, KOD expression was sufficient to cause death in leaves or seedlings and to activate caspase-like activities. In addition, KOD-induced PCD required light in leaves and was repressed by the PCD-suppressor genes AtBax inhibitor 1 and p35. KOD expression resulted in depolarization of the mitochondrial membrane, placing KOD above mitochondria dysfunction, an early step in plant PCD. A KOD∷GFP fusion, however, localized in the cytosol of cells and not mitochondria. |
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Keywords: | BAX inhibitor 1 caspase‐like embryogenesis p35 |
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