Simplified typing of mouse hemoglobin (Hbb) phenotypes using cystamine |
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Authors: | J Barry Whitney III |
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Institution: | (1) The Jackson Laboratory, 04609 Bar Harbor, Maine |
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Abstract: | Cellulose acetate electrophoresis of mouse hemoglobins modified with the disulfide reagent cystamine permits rapid, unequivocal discrimination of all combinations of the codominant mouse hemoglobin single (Hbb
s
) and diffuse (Hbb
d
and Hbb
p
) alleles. The single, diffuse major, diffuse d-minor, and diffuse p-minor adult hemoglobins are all resolved by this method, which depends on the presence of a cysteine in the chains of diffuse mice which is not found in the chain of single mice.This work was supported by research grants ACS-VC58 and NIH CA-01074. The Jackson Laboratory is fully accredited by the American Association for Accreditation of Laboratory Animal Care. |
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Keywords: | hemoglobin electrophoresis Mus musculus polymorphism alkylation |
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