Simplified typing of mouse hemoglobin (Hbb) phenotypes using cystamine |
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Authors: | J. Barry Whitney III |
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Affiliation: | (1) The Jackson Laboratory, 04609 Bar Harbor, Maine |
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Abstract: | Cellulose acetate electrophoresis of mouse hemoglobins modified with the disulfide reagent cystamine permits rapid, unequivocal discrimination of all combinations of the codominant mouse hemoglobin single (Hbbs) and diffuse (Hbbd and Hbbp) alleles. The single, diffuse major, diffuse d-minor, and diffuse p-minor adult hemoglobins are all resolved by this method, which depends on the presence of a cysteine in the chains of diffuse mice which is not found in the chain of single mice.This work was supported by research grants ACS-VC58 and NIH CA-01074. The Jackson Laboratory is fully accredited by the American Association for Accreditation of Laboratory Animal Care. |
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Keywords: | hemoglobin electrophoresis Mus musculus polymorphism alkylation |
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