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Microsatellite markers for the giant kelp Macrocystis pyrifera |
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| Authors: | Filipe Alberto Allison Whitmer Nélson C Coelho Mackenzie Zippay Elena Varela-Alvarez Peter T Raimondi Daniel C Reed Ester A Serrão |
| Institution: | 1. CCMAR, CIMAR-Laboratório Associado, University of Algarve, Campus de Gambelas, Faro, Portugal 2. Marine Science Institute, University of California, Santa Barbara, CA, 93111, USA 3. Department of Ecology and Evolutionary Biology, Center for Ocean Health, Long Marine Lab, University of California, 100 Shaffer Road, Santa Cruz, CA, 95060, USA |
| Abstract: | We report the isolation and characterization of 16 microsatellite loci to study the population genetics of the giant kelp, Macrocystis pyrifera. Markers were obtained by screening a genomic library enriched for microsatellite motifs. Of the 37 primer pairs defined, 16 amplified clean polymorphic microsatellites and are described. These loci identified a number of alleles ranging from three to forty (mean = 16.5, and gene diversity ranging from 0.469 to 0.930 (mean = 0.774). The isolation and characterization of these highly polymorphic markers will greatly benefit much needed studies on the molecular ecology of this important macroalga. |
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