Modern geochemical and molecular tools for monitoring in-situ biodegradation of MTBE and TBA |
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Authors: | Tomasz Kuder Paul Philp |
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Institution: | (1) School of Geology and Geophysics, University of Oklahoma, 100 East Boyd Street Suite 810, Norman, OK 73019, USA |
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Abstract: | Methyl tert-butyl ether (MTBE) is a major gasoline oxygenate worldwide and a widespread groundwater contaminant. Natural attenuation
of MTBE is of practical interest as a cost effective and non-invasive approach to remediation of contaminated sites. The effectiveness
of MTBE attenuation can be difficult to demonstrate without verification of the occurrence of in-situ biodegradation. The
aim of this paper is to discuss the recent progress in assessing in-situ biodegradation. In particular, compound-specific
isotope analysis (CSIA), molecular techniques based on nucleic acids analysis and in-situ application of stable isotope labels
will be discussed. Additionally, attenuation of tert-butyl alcohol (TBA) is of particular interest, as this compound tends to occur alongside MTBE introduced from the gasoline
or produced by (mainly anaerobic) biodegradation of MTBE. |
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Keywords: | Methyl tert-butyl ether (MTBE) Tert-butyl alcohol (TBA) Natural attenuation Biodegradation Groundwater Stable isotope analysis Stable isotope tracers CSIA Molecular tools Bio-Sep |
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