Reconsideration of pollen dispersal data from field trials of transgenic potatoes |
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Authors: | A. J. Conner P. J. Dale |
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Affiliation: | (1) New Zealand Institute for Crop & Food Research, Private Bag 4704, Christchurch, New Zealand;(2) Department of Plant Science, Lincoln University, P.O. Box 84, Canterbury, New Zealand;(3) John Innes Centre, Colney Lane, NR4 7UJ Norwich, UK |
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Abstract: | During the initial field evaluation of transgenic plants, it is usual to isolate them genetically from other plants of the same species. Several field experiments on potatoes, using transgenes as markers, have shown that transgene dispersal by pollen to other potato plants is limited and very unlikely at distances over 10 m. In a recent study in Sweden, a frequency of transgene-containing progeny of over 30% is reported from non-transgenic potato plants grown at distances of 10–1000 m from transgenic plants containing nptII and gus marker genes. Data from the Swedish study is discussed along with other relevant observations, and it is concluded that the high frequency of gene dispersal in that study results from a high frequency of false positives during PCR analysis of the nptII gene. From the data available in potato, it is concluded that a distance of 20 m is generally adequate for the initial field evaluation of transgenic potatoes containing novel gene constructs. |
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Keywords: | Solanum tuberosum Transformation Transgene dispersal Pollination distance Risk assessment |
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