The effect of Ptilotus plant tissue on pH of in vitro media |
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| Authors: | Richard R Williams Acram M Taji Kelly A Winney |
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| Institution: | (1) Department of Agronomy and Soil Science, University of New England, 2351 Armidale, NSW, Australia;(2) Department of Botany, University of New England, 2351 Armidale, NSW, Australia |
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| Abstract: | Changes occur in the pH of in vitro nutrient media during preparation and over the culture period. The direction and extent of the changes depend upon the initial pH and the presence or type of gelling agent. Agar-based medium was progressively acidified in the presence of a living Ptilotus exaltatus explant. This was not a response to wounding and the rate of acidification could be maintained by frequent replacement of the medium. The rate appears to be pH dependent with an equilibrium occurring at pH4. The pH effect was not accounted for by K+ uptake. |
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| Keywords: | autoclaving gelling agent Ptilotus exaltatus wounding |
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