A different approach to generating the data for parameter estimation with the heterotrophic activity method

This paper outlines a different approach to generating the data for V_max and T_t estimation with the Wright-Hobbie [1] method of measuring heterotrophic activities in aquatic environments. To be certain that the incubation times chosen are appropriate for all concentrations of substrate tested, and to increase the precision of the kinetic parameter estimates, we have adopted the approach of using kinetic plots derived from independent time-course studies performed at each concentration of substrate and analyzed by non-linear regression analysis. In keeping with our interest in the impact of acidification on aquatic microbial activities, we have applied this approach to the sediments and water column of the acid-stressed Silver Lake.