Enhancement of stability of biosurfactant produced by <Emphasis Type="Italic">Candida lipolytica</Emphasis> using industrial residue as substrate |
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Authors: | R D Rufino L A Sarubbo G M Campos-Takaki |
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Institution: | (1) Fungal Biology, Federal University of Pernambuco, Recife-Pernambuco, Brazil;(2) Chemistry Department, Catholic University of Pernambuco, Rua do Príncipe, n. 526, Boa Vista, CEP 50.050-900 Recife-PE, Brazil;(3) Nucleus of Research in Environmental Sciences (NPCIAMB), Catholic University of Pernambuco, Rua Nunes Machado, no. 42, Bl J, Boa Vista, 50.050-590 Recife-PE, Brazil |
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Abstract: | This work describes experimental results carried out on the fermentation of Candida lipolytica, which produced a new biosurfactant when grown on a vegetable oil refinery residue as substrate. The cell-free culture broth
containing the biosurfactant formed stable emulsions with hydrophobic natural compounds. Emulsification properties of the
biosurfactant were not affected by salinity; however, treatment at a higher temperature decreased the emulsification activity,
indicating applications in oil recovery. The isolated biosurfactant corresponds to a yield of 4.5 g/l, and the surface tension
of water was reduced from 71 to 32 mN/m. Preliminary chemical characterizations showed that the biosurfactant consisted of
protein (50%), lipid (20%), and carbohydrate (8%). |
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Keywords: | Biosurfactant Candida lipolytica Industrial residue Stability |
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