Isozyme polymorphisms within Pyrenophora tritici-repentis population in Argentina |
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Authors: | María Virginia Moreno María S. Tacalitti Ana M. Castro Analía E. Perelló |
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Affiliation: | (1) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina;(2) Laboratorio de Biología Funcional y Biotecnología (BIOLAB), Facultad de Agronomía de Azul, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN), República de Italia N° 780, Azul, Buenos Aires, 7300, Argentina;(3) Genética, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata (UNLP), Buenos Aires, Argentina;(4) Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de la Plata (UNLP), Buenos Aires, Argentina |
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Abstract: | Tan spot caused by Pyrenophora tritici-repentis is an economically important disease of wheat (Triticum aestivum L.) in Argentina. The success of local breeding programs for resistance to the disease depends to a large extent on the genetic variation within the pathogen population. To assay this variability, 155 isolates of P. tritici-repentis obtained from leaves of wheat and Festuca sp. of different localities were assayed and compared for isozyme polymorphisms. Isozyme analysis was performed with polyacrilamide gel electrophoresis in the systems estearase, phosphatase and peroxidase. Variation in the number and relative mobility of the bands by each system was detected in this collection of isolates. Intraspecific polymorphisms were identified in the three systems assayed. The cluster analysis based in bands patterns defined 86 electromorph types among the 155 isolates evaluated according with the Jaccard’s coefficient (CCC = 095). The isozymes patterns were not correlated with the geographic and/or wheat cultivars origin of the P. tritici-repentis isolates. |
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Keywords: | Electrophoretic Isozyme Polymorphisms Pyrenophora tritici-repentis Variability Wheat |
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