Preliminary analysis of the cauliflower mitochondrial proteome |
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| Authors: | T?Pawlowski M?Rurek S?Janicka K?D?Raczynska Email author" target="_blank">H?AugustyniakEmail author |
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| Affiliation: | (1) Institute of Dendrology of the Polish Academy of Sciences, 5 Parkowa Str., 62-035 Kórnik, Poland;(2) Department of Plant Molecular Biology, Institute of Molecular Biology and Biotechnology, A. Mickiewicz University, 5 Mi dzychodzka Str., 60-371 Pozna, Poland |
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| Abstract: | Purified cauliflower (Brassica oleracea var. botrytis) mitochondrial proteins fractionated into soluble, membrane, integral membrane and peripheral membrane samples were analyzed
by 2D- PAGE (isoelectric focusing/ SDS polyacrylamide gel electrophoresis). 2D gels patterns were compared using the Imager
Master 2D Elite software. 561 silver stained protein spots were resolved after electrophoresis under standard conditions of
a whole protein extract. In the soluble fraction a prevalent number of more intense protein spots was observed. The cauliflower
protein 2D patterns resembled Arabidopsis thaliana 2D patterns. The two protein spots selected which occupied a similar isoelectric point positions on both gels represented
the same proteins as revealed by ESI-MS analysis of cauliflower proteins. The third selected spot belongs to unidentified
proteins. The comparative analysis of mitochondrial suborganellar fractions proved the usefulness of this approach. |
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| Keywords: | Cauliflower mitochondria Fractionation Two dimensional gel electrophoresis |
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