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Essential arginine residues in beef kidney D-aspartate oxidase (a preliminary report)
Authors:Carlo Crifò  Luigi Santoro  Augusto Rinaldi  Carlo de Marco
Affiliation:(1) Istituto di Chimica Biologica della Università di Cagliari, Cagliari, Italy;(2) I Cattedra di Chimica Biologica della Facoltà di Scienze M.F.N. della Università di Roma, Roma, Italy
Abstract:Summary Partially purifiedD-aspartate oxidase from beef kidney has been tested in the presence of butanedione or phenylglyoxal, which specifically modify the arginine molecule. The results obtained clearly indicate that arginine residues are involved in the binding of the substrate to the active site of the enzyme.
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