Asymmetric segregation of the complementary sex-factor DNA strands during conjugation in Escherichia coli |
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| Authors: | D Vapnek W D Rupp |
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| Affiliation: | 1. Yersinia Research Unit, Institut Pasteur, F-75724, Paris, France;2. Université Paris-Diderot, Sorbonne Paris Cité, F-75013, Paris, France;3. National Reference Laboratory ‘Plague & Other Yersiniosis’, Institut Pasteur, F-75724, Paris, France;4. World Health Organization Collaborating Research & Reference Centre for Yersinia, Institut Pasteur, F-75724, Paris, France;1. Department of Microbiology and Immunology, Ross University School of Medicine, North Brunswick, NJ, USA;1. Department of Infection, Guy’s and St Thomas’ NHS Foundation Trust, London, UK;2. Centre for Clinical Infection & Diagnostics Research (CIDR), King’s College London, London, UK;3. UK Health Security Agency – Colindale;4. Medical Research Council, Clinical Trials Unit, University College London, London, UK |
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| Abstract: | When breaks are introduced into double-stranded covalently closed circular sex-factor DNA molecules, the complementary strands can be separated in a CsCl-poly (U, G) equilibrium density gradient. Using this technique, double-stranded circular sex-factor DNA isolated from donor and recipient cells after mating was analyzed to determine the fate of the complementary strands.Only one strand of the sex-factor DNA is transferred from donor to recipient cells, this being the denser strand in CsCl-poly (U, G). A complement to this strand is synthesized in the recipient and a covalently closed sex-factor DNA molecule is formed. The sex-factor strand not transferred to the recipient remains in the donor where it acquires a complement during mating and forms a covalently closed double-stranded circle.These results show that DNA synthesis associated with mating occurs in both the donor and recipient cells. The asymmetric segregation of the complementary sex-factor DNA strands could be generated by a mechanism such as the rolling circle model of DNA synthesis proposed by Gilbert &; Dressler (1968). |
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