An optimum environment for the culturing of cytospora isolates from stone fruits. II. Carbon sources

Summary Six isolates (Cytospora cinctaFr. andC. leucostomaFr.) were innoculated to liquid and solid synthetic laboratory media containing nine different sources of carbon.One of the isolates did not grow. The remaining five grew at rates, and in relationships, which segregated them in accordance with their species grouping. These relationships were more nearly constant than any other relationships previously noted in Idaho for these or otherCytopora isolates.Two conclusions were reached: 1) For best growth and sporulation, maltose should be incorporated in the laboratory culture medium and not the commonly used dextrose, which was demonstrated to be an inferior source of carbon; and 2) separate investigations, which utilize different carbon sources in the laboratory media, are likely to yield sufficiently different results that co-identity of species strains used by the separate workers may not be evident.Approved by the Director of the Idaho Agricultural Experiment Station as Research Paper No. 489.