Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila
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| Authors: | Pavan Ramdya Thomas Schaffter Dario Floreano Richard Benton |
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| Institution: | 1. Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.; 2. Laboratory of Intelligent Systems, Institute of Microengineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.; Center for Genomic Regulation, Spain, |
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| Abstract: | Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila. |
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