Chemotactic Behavior of Pathogenic and Nonpathogenic Leptospira Species |
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| Authors: | Ambroise Lambert Naoko Takahashi Nyles W. Charon Mathieu Picardeau |
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| Affiliation: | aInstitut Pasteur, Unité de Biologie des Spirochètes, Paris, France;bUniversité Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France;cDepartment of Microbiology, Immunology, and Cell Biology, West Virginia University, Health Sciences Center, Morgantown, West Virginia, USA |
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| Abstract: | We have developed a capillary tube assay in combination with real-time PCR to quantitate the number of chemoattracted Leptospira cells. We identified Tween 80, glucose, sucrose, and pyruvate as attractants for Leptospira cells; amino acids and vitamin B12 were found to be nonchemotactic or weakly chemotactic. This assay has the general applicability to further our understanding of leptospiral chemotaxis. |
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