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Impact of Sorbic Acid on Germination and Outgrowth Heterogeneity of Bacillus cereus ATCC 14579 Spores |
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| Authors: | Heidy M W den Besten Clint C J van Melis Jan Willem Sanders Masja N Nierop Groot Tjakko Abee |
| Institution: | aWageningen University, Laboratory of Food Microbiology, Wageningen, the Netherlands;bTI Food and Nutrition, Wageningen, the Netherlands;cUnilever Research and Development, Biosciences, Vlaardingen, the Netherlands;dWageningen University and Research Centre, Food and Biobased Research, Wageningen, the Netherlands |
| Abstract: | Population heterogeneity complicates the predictability of the outgrowth kinetics of individual spores. Flow cytometry sorting and monitoring of the germination and outgrowth of single dormant spores allowed the quantification of acid-induced spore population heterogeneity at pH 5.5 and in the presence of sorbic acid. This showed that germination efficiency was not a good predictor for heterogeneity in final outgrowth. |
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