Heme oxygenase-1 induction by endogenous nitric oxide: influence of intracellular glutathione |
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Authors: | André Muriel Felley-Bosco Emanuela |
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Institution: | Institute of Pharmacology and Toxicology, Rue du Bugnon 27, 1005 Lausanne, Switzerland. |
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Abstract: | To investigate the influence of glutathione (GSH) on cellular effects of nitric oxide (NO) formation, human colon adenocarcinoma cells were transfected with a vector allowing controlled expression of inducible nitric oxide synthase (iNOS). Protein levels of oxidative stress-sensitive heme oxygenase-1 (HO-1) were analyzed in the presence or absence of GSH depletion using L-buthionine-S,R]-sulfoximine and iNOS induction. While no effect was observed in the presence of iNOS activity alone, a synergistic effect on HO-1 expression was observed in the presence of iNOS expression and GSH depletion. This effect was prevented by addition of N-methyl-L-arginine. Therefore, targeting of endogenous NO may be modulated by intracellular GSH. |
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