Construction and properties of a new cloning vehicle, allowing direct sereening for recombinant plasmids |
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Authors: | Ulrich Rü ther |
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Affiliation: | (1) Institute for Genetics, University of Cologne, Weyertal 121, D-5000 Cologne 41, Federal Republic of Germany |
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Abstract: | Summary pUR2, a certified B2(EK2) vector, allows easy isolation of variants containing cloned EcoRI-fragments. Bacteria harboring plasmids without inserts make blue colonies on indicator-plates, whilst those harboring recombinant plasmids make white colonies. |
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