Partial characterization of extracellular polysaccharides produced by cyanobacterium Arthrospira platensis |
| |
| Authors: | Lamia Trabelsi Nour Houda M’sakni Hatem Ben Ouada Hassen Bacha Sadok Roudesli |
| |
| Institution: | (1) Laboratory of Marine Biodiversity and Biotechnology, National Institute of Marine Sciences and Technology, Monastir, 5000, Tunisia;(2) Laboratory of Polymers, Bio-polymers and Organic Materials, Faculty of Sciences, Monastir, 5019, Tunisia;(3) Laboratoire de Recherches sur les Substances Biologiquement Compatibles, Faculte de Medecine Dentaire, Monastir, 5000, Tunisia |
| |
| Abstract: | In this study, the physico-chemical characteristics of extracellular polysaccharides (EPS) produced by Arthrospira platensis were evaluated. Elemental analysis and a bicinchoninic acid (BCA) reaction indicated that the EPS were heteropolysaccharides
that contain carbohydrate (13%) and protein (55%) moieties. Analysis of the infrared spectrum and elemental analysis revealed
the presence of a sulfate group (0.5%). The UV-visible spectrum showed high UV absorption at 190∼230 nm and a shoulder at
260∼280 nm. In addition, this spectrum indicated that EPS can form aggregates with mycosporine-like amino acids and/or scytonemin.
Gas chromatography analysis of the carbohydrate portion of the EPS indicated that it was composed of seven neutral sugars:
galactose (14.9%), xylose (14.3%), glucose (13.2%), frucose (13.2%), rhamnose (3.7%), arabinose (1%), and mannose (0.3%) and
two uronic acids, galacturonic acid (13.5%) and glucuronic acid (0.9%). |
| |
| Keywords: | Arthrospira platensis cyanobacteria heteropolysaccharides physico-chemical characterization |
| 本文献已被 SpringerLink 等数据库收录！ |
|
