生长素与乙烯在沙田柚上胚轴不定芽再生中的作用

结果表明,6 BA只能诱导较低频率的不定芽再生,IBA的加入可以促进不定芽的再生。从再生率与单位外植体再生芽数综合考虑,以高浓度IBA(1.5mg·L 1)与低浓度6 BA(0.5mg·L 1)配合的效果最佳,加入生长素极性运输的调节剂TIBA与Flavone可进一步提高不定芽的再生频率。乙烯抑制上胚轴不定芽的再生,而乙烯生理作用的拮抗剂Ag+与生物合成抑制剂AOA、Co+可以显著提高不定芽再生频率。水杨酸(SA)也抑制不定芽的再生。

Roles of auxin and ethylene in direct bud regeneration from epicotyl of Citrus grandis(L.) Osbeck cv. Shatian Yu

The roles of auxin and ethylene in direct bud regeneration from epicotyl of Citrus grandis(L.)Osbeck cv. Shatian Yu were investigated. The results showed that 6 BA could not induce bud regeneration efficiently. The addition of IBA could promote it synergistically. The best results was obtained with the combination of 1.5 mg·L 1 IBA and 0.5 mg·L 1 6 BA in terms of regeneration frequency and regenerated shoot number per explant. The auxin polar transport regulators,TIBA and Flavone,could further improve regeneration frequency. Ethylene inhibited shoot regeneration,while its antagonist,Ag+,and synthetic inhibitors,AOA and Co+,could significantly improve regeneration frequency. SA also inhibited shoot regeneration.