| 大鼠精核蛋白的纯化 |
| |
| 引用本文： | 费仁仁,戴文平,戴巾,陈晖,朱斌,邓锦松.大鼠精核蛋白的纯化[J].中国生物化学与分子生物学报,1994,10(5):557-560. |
| |
| 作者姓名： | 费仁仁 戴文平 戴巾 陈晖 朱斌 邓锦松 |
| |
| 作者单位： | 中国医学科学院药物研究所，中国医学科学院基础医学研究所，美国北卡罗来纳州立大学 |
| |
| 摘 要： | 用大鼠睾丸制备长形精子细胞核及用附睾制备的附睾精子核，用盐酸胍法提取总碱性蛋白（Ｔｏｔａｌ ｂａｓｉｃ ｐｒｏｔｅｉｎ，ＴＢＰ）。通过Ｓｅｐｈａｄｅｘ Ｇ－１００分子筛层析，其第三峰用５％ＴＣＡ沉淀，得到纯大鼠精核蛋白（Ｒａｔ ｐｒｏｔａｍｉｎｅ，ＲＰ）。ＲＰ也可用ＴＢＰ通过ＨＰＬＣ反相柱得到纯化，ＲＰ位于２６％－２８％乙腈梯度。
|
| 关 键 词： | 精核蛋白 精子发生 精子细胞 |
| 收稿时间： | 1994-10-20 |
Purification of Rat Protamine |
| |
| Fei,Ren-ren,Dai,Wen-ping,Dai,jin,Chen,Hui,Zhu,Bin,Teng,Ching-sung.Purification of Rat Protamine[J].Chinese Journal of Biochemistry and Molecular Biology,1994,10(5):557-560. |
| |
| Authors: | Fei Ren-ren Dai Wen-ping Dai jin Chen Hui Zhu Bin Teng Ching-sung |
| |
| Institution: | (Chinese Academy of Medical Sciences, Beijing 100005 |
| |
| Abstract: | The acid-soluble proteins were extracted from testis elongated spermatid nuclei and epididymal sperm nuclei using guanidine-HCl and applied to a column of Sephadex G-100. Protamine was purified from the fraction of gel filtration chromatography by precipitation with 5% TCA. Rat protamine can also be purified through a reverse-phase HPLC. The protamine was located in the eluate of 26% to 28% acetonitrile. |
| |
| Keywords: | Protamine Spermatogenesis Spermatid |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息 |
| 点击此处可从《中国生物化学与分子生物学报》下载免费的PDF全文 |
