| 鸡肠中氨基酸含量的高效液相色谱分析 |
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| 引用本文： | 尚素芬,王洪.鸡肠中氨基酸含量的高效液相色谱分析[J].氨基酸和生物资源,1997,19(1):22-24,27. |
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| 作者姓名： | 尚素芬 王洪 |
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| 作者单位： | 河北大学化学系 |
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| 摘 要： | 本文采用以异硫氰酸苯酯（ＰＩＴＣ）为柱前衍生化的高效液相色谱法测定氨基酸，并对方法测定条件进行了研究，得出流动相ｐＨ６．４０时测定的ＲＳＤ值最低，为０．５９％，样品经微波水解７ｍｉｎ（３００Ｗ功率），且稀释液加入５ｍｉｎ后进样方能得到最佳结果。用本法对鸡肠中氨基酸进行了分析，必需氨基酸总量占３４．９％，具有可开发利用之价值。
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| 关 键 词： | 氨基酸，鸡肠，高效液相色谱 |
Analysis of Amino Acid in Chicken Intestines with High Performance Liquid Chromatography |
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| Shang Sufen,Wang Hong.Analysis of Amino Acid in Chicken Intestines with High Performance Liquid Chromatography[J].Amino Acids & Biotic Resources,1997,19(1):22-24,27. |
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| Authors: | Shang Sufen Wang Hong |
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| Institution: | Shang Sufen Wang Hong Department of Chemistry,He bei University,Baoding,071002 |
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| Abstract: | Analysis of amino acids by the HPLC Pico-Tag method was studied in this paper. The procedure for analysis was optimized. It was found that the pH value of mobile phase was 6.40 with a RSD of 0.59%, and reliable results were obtained by injecting the sample after 5 minutes of adding sample diluent solution. The content of amino acids in chicken intestines was also determined and the content of essential amino acids was 34.9%. This method was rapid, sensitive, accurate and reproducible. |
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| Keywords: | HPLC Pico-Tag Amino aeid Chicken intestines |
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