Growth Response of Axenic Entamoeba histolytica to Hydrogen, Carbon Dioxide, and Oxygen *,† |
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| Authors: | R. NEAL BAND HELEN CIRRITO |
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| Affiliation: | Department of Zoology, Michigan State University, East Laming, Michigan 48824 |
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| Abstract: | Entamoeba histolytica required CO2 for growth in axenic culture while growth was inhibited by H2. The organism was tolerant to 5% O2 in the gas phase and it was able to detoxify products of O2 reduction in the medium. The ameba did not require a negative oxidation-reduction potential for axenic growth. However, little or no free O2 was present in media exposed to 5% O2 in the gas phase. Growth was improved by adding yeast extract to the medium. |
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| Keywords: | Index Key Words: Entamoeba histolytica: axenic culture growth H2 CO2 O2 oxidation-reduction potential. |
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