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Mobilization of cloned luciferase genes into Vibrio harveyi luminescence mutants |
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| Authors: | Subhash C. Gupta Catherine Potrikus Reese J. Woodland Hastings |
| Affiliation: | (1) Department of Cellular and Developmental Biology, Harvard University, 16 Divinity Avenue, 02138 Cambridge, MA, USA;(2) Present address: Ph.D.-M.D. Program, University of Miami, 33125, FL, USA;(3) Present address: Pfizer Central Research, Eastern Point Road, 06340 Groton, CT, USA |
| Abstract: | A recombinant plasmid which carried a 5 kb fragment of Vibrio harveyi DNA containing the luxA and luxB genes was mobilized from Escherichia coli into luminescence-deficient mutants of V. harveyi. The cloned genes complemented a temperature sensitive luciferase mutation, but failed to complement lesions in two different aldehyde deficient mutants. Expression of the cloned genes was not subject to autoinduction in either E. coli or in V. harveyi. |
| Keywords: | Plasmid mobilization Bioluminescence Temperature conditional Complementation Marine bacteria Autoinduction Vibrio harveyi Luciferase genes Cloning |
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