Sex identification of owls (family Strigidae) using oligonucleotide microarrays |
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Authors: | Wang Lih Chiann Severinghaus Lucia Liu Chen Chi Tsong Liu Lu Yuan Pan Chu Hsiang Huang Dean Lee Hsiao Yuan Lir Jihn Tsair Chin Shih Chien Pu Chang En Wang Ching Ho |
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Institution: | Graduate Institute of Veterinary Medicine, National Taiwan University, 1 Sec. 4, Roosevelt Road, Taipei 106, Taiwan. |
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Abstract: | Molecular sexing of the diversified avian family Strigidae is difficult. Sex identification using the intron length difference between W and Z chromosomal CHD1 genes, as visualized by agarose gel electrophoreses, often produces ambiguous results. Here we describe a simple method for sexing a variety of Strigidae species using oligonucleotide microarrays, on which several sex-specific probes operated complementarily or in concert. The sex of 8 owl species was identified clearly on the microarrays through sequence recognition. This sequence-directed method can be easily applied to a wider range of Strigidae species. |
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