Phosphofructokinase 2: the enzyme that forms fructose 2,6-bisphosphate from fructose 6-phosphate and ATP |
| |
| Authors: | E Van Schaftingen H G Hers |
| |
| Affiliation: | 1. Laboratoire de Physiologie Cellulaire (ERA 323), Universite Pierre et Marie Curie, 4 place Jussieu 75005 Paris-France;2. Laboratoire de Biochimie Végétale (ERA 487), Institut de Botanique, 28 rue Goethe, 67083 Strasbourg-France |
| |
| Abstract: | The polypeptide composition of plasmalemma, mitochondria and endoplasmic reticulum, isolated from maize coleoptile, was determined using polyacrylamide gel electrophoresis in the presence of lithium dodecyl sulfate. A polypeptide with an apparent molecular weight of 90,000 was found to be the major polypeptide associated with plasmalemma. This polypeptide is firmly attached to the membrane since it was not extracted by KCl. However, after a treatment with Triton X100, the polypeptide was detached from the membrane. It was also found that a heat treatment of the membrane partly destroyed this polypeptide. This effect of heat treatment was also observed after isolation of the polypeptide by electrophoresis. |
| |
| Keywords: | PFK phosphofructokinase Hepes 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid bis-tris propane 1,3-bis[tris(hydroxymethyl)-methylamino]-propane EGTA ethyleneglycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
