Modulation of penicillin acylase properties via immobilization techniques: one-pot chemoenzymatic synthesis of Cephamandole from Cephalosporin C. |
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Authors: | M Terreni G Pagani D Ubiali R Fernández-Lafuente C Mateo J M Guisán |
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Institution: | Pharmaceutical Biocatalysis Laboratories, Dipartimento di Chimica Farmaceutica, Università degli Studi di Pavia, via Taramelli 12, 27100, Pavia, Italy. markt@pbl.unipv.it |
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Abstract: | The modulation of penicillin G acylase (PGA) properties via immobilization techniques has been performed studying the acylation of 7-aminocephalosporanic acid with R-mandelic acid methyl ester. PGA from Escherichia coli, immobilized onto agarose activated with glycidol (glyoxyl-agarose), has been used for the design of a novel one-pot synthesis of Cephamandole in aqueous medium and without isolation of intermediates, through three consecutive biotransformations catalyzed by D-amino acid oxidase, glutaryl acylase and PGA. |
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