Genotype-independent leaf disc transformation of potato (Solanum tuberosum) using Agrobacterium tumefaciens |
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Authors: | M De Block |
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Institution: | (1) Plant Genetic Systems, Jozef Plateaustraat 22, B-9000 Gent, Belgium |
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Abstract: | Summary Leaves of the in vitro grown potato cultivars Bintje , Berolina , Desiree , and Russet Burbank were wounded and co-cultivated with Agrobacterium strains having chimeric bar and nptII genes on a disarmed T-DNA. Each leaf from these cultivars formed numerous calli on kanamycin-containing medium, and almost all calli regenerated shoots. For Russet Burbank , it was necessary to include AgNO3 in the medium to obtain efficient shoot regeneration. The transformed plants have one to a few copies of the T-DNA, show NPT-II and PAT activities, and are resistant to high doses of the commercial preparation of phospinotricin (glufosinate). Almost no somaclonal variation was detected in trans-genic plants. |
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Keywords: | Solanum tuberosum Transformation Agrobacterium Ethylene Phosphinotricin |
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