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Genotype-independent leaf disc transformation of potato (Solanum tuberosum) using Agrobacterium tumefaciens
Authors:M De Block
Institution:(1) Plant Genetic Systems, Jozef Plateaustraat 22, B-9000 Gent, Belgium
Abstract:Summary Leaves of the in vitro grown potato cultivars lsquoBintjersquo, lsquoBerolinarsquo, lsquoDesireersquo, and lsquoRusset Burbankrsquo were wounded and co-cultivated with Agrobacterium strains having chimeric bar and nptII genes on a disarmed T-DNA. Each leaf from these cultivars formed numerous calli on kanamycin-containing medium, and almost all calli regenerated shoots. For lsquoRusset Burbankrsquo, it was necessary to include AgNO3 in the medium to obtain efficient shoot regeneration. The transformed plants have one to a few copies of the T-DNA, show NPT-II and PAT activities, and are resistant to high doses of the commercial preparation of phospinotricin (glufosinate). Almost no somaclonal variation was detected in trans-genic plants.
Keywords:Solanum tuberosum  Transformation  Agrobacterium  Ethylene  Phosphinotricin
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