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Immunocytochemical detection of ribulose bisphosphate carboxylase in capsicum plastids
Authors:Catherine Cheniclet  Claude Suire  Jean-Pierre Carde
Affiliation:1. Department of Chemistry in Pharmaceutical Sciences, Analytical Chemistry, Faculty of Pharmacy, Universidad Complutense de Madrid (UCM), Avenida Complutense, Madrid (28040). VALORNUT Research Group (920030-UCM), Spain;2. Department of Metabolism and Nutrition, Institute of Food Science, Technology and Nutrition (ICTAN-CSIC), José Antonio Novais 10, 28040 Madrid, Spain;1. University of Panamá, Biochemistry and Nutrition Laboratory, Panama City, Panama;2. University of Pécs, Medical School, Department of Biochemistry and Medical Chemistry, Szigeti út 12, H-7624, Pécs, Hungary;3. University of Pécs, Medical School, Institute of Bioanalytics, Szigeti út 12, H-7624, Pécs, Hungary;4. University of Pécs, Faculty of Pharmacy, Department of Pharmacognosy, Rókus utca 2, H-7624, Pécs, Hungary;1. Center for Molecular Imaging Probe, Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China;2. Department of Pharmacy and Pharmacology, Hengyang Medical School, University of South China, Hengyang, China;3. Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang, China
Abstract:Ribulose bisphosphate carboxylase (RUBPCase) was localized by fluorescence and gold immunocytochemistry in Capsicum fruits. Chloroplasts of the green fruit are heavily labelled. A positive staining is also obtained with chromoplasts of the ripe rad fruit, but gold labelling is fainter. The presence of reactive RuBPCase in chromoplasts is discussed in relation with the absence of ribosomes in these plastids.
Keywords:chloroplasts  chromoplasts  immunocytochemistry  Ribulose bisphosphate carboxylase
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