| A群脑膜炎球菌多糖-破伤风类毒素结合物中游离多糖测定方法的建立 |
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| 引用本文： | 朱为,余生玲,朱文瑛,王晓,黄帼英,李凤祥,李亚楠. A群脑膜炎球菌多糖-破伤风类毒素结合物中游离多糖测定方法的建立[J]. 微生物学免疫学进展, 2002, 30(4): 29-32 |
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| 作者姓名： | 朱为 余生玲 朱文瑛 王晓 黄帼英 李凤祥 李亚楠 |
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| 作者单位： | 1. 上海生物制品研究所,上海,200052
2. 中国药品生物制品检定所 |
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| 摘 要： | 本文建立一种测定A群脑膜炎球菌多糖－破伤风类毒素（PS－TT）结合物中游离多糖的方法。用80％乙醇使PS－TT结合物沉淀，再用60％乙醇洗涤沉淀使未结合多糖溶解，使与TT结合的和未结合的多糖分离并分别测定。该方法可有效分离结合的与未结合的多糖并分别测定，结合物中外加多糖可定量回收。本方法适用于测定A群脑膜炎球菌多糖－破伤风类毒素结合物中游离多糖含量。
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| 关 键 词： | 破伤风类毒素 测定方法 A群脑膜炎球菌 多糖结合物 游离多糖 |
| 文章编号： | 1005-5673(2002)-04-0029-04 |
| 修稿时间： | 2002-01-04 |
A method for quantitative test of unconjugated meningoccocal polysaccharide(PS) in PS-tetanus toxoid conjugate preparations |
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| ZHU Wei ,YU Sheng-ling ,ZHU Wen-ying ,et al.. A method for quantitative test of unconjugated meningoccocal polysaccharide(PS) in PS-tetanus toxoid conjugate preparations[J]. Progress In Microbiology and Immunology, 2002, 30(4): 29-32 |
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| Authors: | ZHU Wei YU Sheng-ling ZHU Wen-ying et al. |
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| Affiliation: | ZHU Wei 1,YU Sheng-ling 1,ZHU Wen-ying 1,et al. |
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| Abstract: | The method for quantitative test of free meningoccocal polysaccharide(PS) in PS-tetanus toxoid conjugate was developed based on the ethanol precipitation of tetanus toxoid(TT).The conjugate was treated with 80% ethanol to precipitate PS-TT, then the precipitant was washed with 60% ethanol to resolve free PS, and to separate unconjugated PS from conjugated PS-TT.This method could separate free PS from conjugated PS-TT effectively and quantitative.The accuracy of the assay was confirmed by spiking known amounts of free PS to PS-TT conjugate preparations. This method is useful for measuring content of unconjugated PS in preparations of meningoccocal PS-TT conjugate. |
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| Keywords: | Meningoccocal Polysaccharide conjugate Free polysaccharide |
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