Chain length characterization of oligodeoxyribonucleotides by analytical ultracentrifugation |
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Authors: | D C Luk P Bartl A L Nussbaum |
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Affiliation: | Chemical Research Department, Hoffmann-La Roche Inc., Nutley, New Jersey 07110 USA |
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Abstract: | Analytical ultracentrifugation was used to determine chain length of oligomers in the deoxyribonucleotide series. Uncertainties with respect to partial specific volume of the solute and questions of charge compel a semiempirical approach: variations of these parameters introduce fluctuations well within the experimental error of the method. Measurements of the sedimentation equilibria for three homooligomer series—d(pT)n, d(pA)n and d(pC)n—as well as a number of oligomers of varying base composition generated straight-line plots passing through the origin. |
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