Effects of parathyroid hormone on cyclic AMP-formation and cytoplasmic free Ca2+ in the osteosarcoma cell line UMR 106-01 |
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Authors: | Östen Ljunggren Hans Johansson Ulf H Lerner Erik Lindh Sverker Ljunghall |
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Institution: | (1) Department of Internal Medicine, University Hospital, S-751 85 Uppsala, Sweden;(2) Department of Surgery, University Hospital, S-751 85 Uppsala, Sweden;(3) Department of Oral Cell Biology, University of Umeå, S-901 87 Umeå, Sweden |
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Abstract: | The effects of parathyroid hormone (PTH) on cytoplasmic free CA2+ (Ca
i
2+
) and cAMP-formation were investigated in the rat osteosarcoma cell line UMR 106-01.In fura-2 loaded adherent single cells bPTH 1-34 (10 nM–1M) induced a rapid transient increase in Ca
i
2+
in 11% of the studied cells. In fura-2 tracings from UMR 106-01 cells in suspension, bPTH 1-34 (0.1 M) induced a transient increase in Ca
i
2+
in 20% of the experiments. The transient increase in Ca
i
2+
seen in suspensions of cells was not abolished by addition of EGTA (2.5 mM) prior to challenge with PTH, suggesting that the increase in Ca
i
2+
was derived from intracellular stores.A marked rapid increase in cAMP-formation was observed in all experiments with cells in suspension, also in the experiments where PTH did not affect Ca
i
2+
.These data show that PTH causes a release of Ca2+ from intracellular stores in a small percentage of osteosarcoma UMR 106-01 cells, and that PTH is capable of inducing an increase in cAMP-formation without affecting Ca
i
2+
in osteoblasts. |
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Keywords: | parathyroid hormone cytoplasmic Ca2+ cyclic AMP osteoblasts |
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