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盐胁迫对甜菊细胞内游离Ca^2+浓度的影响(简报)
引用本文:熊玲媛,徐金森,等.盐胁迫对甜菊细胞内游离Ca^2+浓度的影响(简报)[J].实验生物学报,2002,35(2):151-154.
作者姓名:熊玲媛  徐金森
摘    要:

关 键 词:盐胁迫  甜菊  细胞内游离Ca^2+浓度  胞质Ca^2+  LSCM  肌醇磷脂调节系统

Effects of salinity stress on cytosolic calciumin Stevia rebaudiana Bertoni cells]
Ling Yuan Xiong,Jin Sen Xu,Fu Xiang Hong,Mu Chuan Chen.Effects of salinity stress on cytosolic calciumin Stevia rebaudiana Bertoni cells][J].Acta Biologiae Experimentalis Sinica,2002,35(2):151-154.
Authors:Ling Yuan Xiong  Jin Sen Xu  Fu Xiang Hong  Mu Chuan Chen
Institution:School of Life Sciences/The Key Laboratory of Education Ministry for Cell Biology and Tumor Cell Engineering, Xiamen University, Xiamen 361005, China.
Abstract:Fluo-3/AM, a calcium indicator, was introduced by low temprature loading method into callus protoplasts of Stevia rebaudiana Bertoni. The microscope observation results showed that NaCl in different concentrations (30-200mmol/L) can elevate the intracellular calcium concentration in protoplasts. The elevation was related to the amount of salinity. The effect of salinity stress was inhibited by LiCl pretreatment but restored by inositol pretreatment. This suggested that salinity stress promoted the cytoplasmic calcium activity, perhaps by activating the phosphoinoditide system. As a result of salinity stress signals, elevated calcium activity may trigger corresponding metabolic changes, such as synthesis of enzyme via activating other members of calcium signal system, fit the cells for the change of the environment.
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