Genetic and molecular investigations on the endogenous mobile elements of non-drosophilid fruitflies |
| |
Authors: | C Torti LM Gomulski AR Malacrida P Capy G Gasperi |
| |
Institution: | (1) Department of Animal Biology, University of Pavia, Pavia, Italy;(2) Génétique et Evolution. CNRS, Laboratoire de Populations, Gif/Yvette, France |
| |
Abstract: | A syndrome of abnormal genetic effects, resembling Drosophila hybrid dysgenesis, occurs in Ceratitis capitata when strains
of different origin are mated. The pattern of abnormal traits observed appears to be the phenotypic expression of a complex
interacting dysgenic system of inducer and suppressor effects; probably more than one system is activated in the crosses.
This suggests that different systems of mobile elements occur in different strains and populations of C. capitata. Using a
PCR primer specific to the ITR sequence of a deleted element, full length mariner elements were isolated from C. capitata,
Ceratitis rosa, and Trirhithrum coffeae. Very high similarities were found in inter- and intraspecific comparisons of the
elements. The majority of these elements contained deletions and frame-shifts. However, one clone Ccmar1.18, from C. capitata,
was found to possess an uninterrupted ORF coding for 338 amino acids with ∼60% similarity to the Mos1 element of Drosophila
mauritiana. Database searches and phylogenetic analyses showed that the mariner elements isolated in the present study are
representatives of Robertson's mellifera mariner subfamily. The copy numbers of the elements within each species are very
different, ranging from about 10 in T. coffeae to 5000 in C. rosa.
This revised version was published online in August 2006 with corrections to the Cover Date. |
| |
Keywords: | Tephritidae flies Ceratitis capitata hybrid dysgenesis mariner-like elements |
本文献已被 SpringerLink 等数据库收录! |
|