| 产脂肪酶深海细菌的筛选鉴定及酶学性质研究 |
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| 引用本文： | 王凯,刘洪国,姜昆,闫培生. 产脂肪酶深海细菌的筛选鉴定及酶学性质研究[J]. 生物技术进展, 2015, 5(3): 241-245. DOI: 10.3969/j.issn.2095-2341.2015.03.16 |
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| 作者姓名： | 王凯 刘洪国 姜昆 闫培生 |
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| 作者单位： | 哈尔滨工业大学（威海）海洋科学与技术学院, 山东 威海 264209 |
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| 基金项目： | 中国大洋协会国际海域资源调查与开发“十二五”重大项目（DY125-15-R-01）；山东省优秀中青年科学家科研奖励基金（BS2014NY012）；哈尔滨工业大学学科建设引导基金（WH20140202）资助。 |
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| 摘 要： | 从分离自南大西洋深海沉积物的细菌中筛选出一株产脂肪酶菌株FE10，通过16S rRNA序列分析，对FE10进行分子鉴定和系统发育分析，初步确定其为海杆菌属（Marinobacter）。对菌株FE10所产脂肪酶进行酶学性质初步研究表明：在实验温度条件下所产脂肪酶40℃、pH 7时酶活最高，pH 9时酶活几乎消失。
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| 关 键 词： | 深海 微生物 脂肪酶 16S rRNA 酶学性质 |
| 收稿时间： | 2015-04-01 |
Study on Classification and Enzymatic Propertis of Deep-sea Bacteria Producing Lipase |
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| WANG Kai,LIU Hong-guo,JIANG Kun,YAN Pei-sheng. Study on Classification and Enzymatic Propertis of Deep-sea Bacteria Producing Lipase[J]. CURRENT BIOTECHNOLOGY, 2015, 5(3): 241-245. DOI: 10.3969/j.issn.2095-2341.2015.03.16 |
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| Authors: | WANG Kai LIU Hong-guo JIANG Kun YAN Pei-sheng |
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| Affiliation: | School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Shandong Weihai 264209, China |
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| Abstract: | The bacterial strain FE10 producing lipase was screened from deep sea bacteria, which isolated from deep-sea sediment of South Atlantic. The molecular identification of 16S rRNA gene sequencing and phylogenetic analysis showed that FE10 belonged to the genus Marinobacter. The results of preliminary study on the lipase characterization showed that optimal temperature of the lipase activity was 40℃ under laboratory conditions. The lipase activity was high at pH 7, and almost disappeared at pH 9. |
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| Keywords: | deep sea microbes lipase 16S rRNA enzymatic characteristics |
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